Composite
IBR-T25
Part:BBa_K3254004:Design
Designed by: Yeqing Zong, Qianrui Li Group: iGEM19_GENAS_China (2019-10-13)
Int10attB-BsaI site-terminator-Int10attP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 76
Illegal BsaI.rc site found at 64
Design Notes
This part sequence was tested in silico before synthesised to avoid potential promoter activity (see the website in references).
Source
This part was totally synthesised by commerical company.